Fetal demise with Smith–Lemli–Opitz syndrome confirmed by tissue sterol analysis and the absence of measurable 7-dehydrocholesterol Δ7-reductase activity in chorionic villi
✍ Scribed by L. M. Linck; S. J. Hayflick; D. S. Lin; K. P. Battaile; S. Ginat; T. Burlingame; K. M. Gibson; M. Honda; A. Honda; G. Salen; G. S. Tint; W. E. Connor; R. D. Steiner
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 51 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0197-3851
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✦ Synopsis
Smith±Lemli±Opitz syndrome (SLOS), an autosomal recessive condition with multiple malformations, mental retardation, and growth failure, results from markedly reduced activity of the ®nal enzyme in the cholesterol biosynthetic pathway, 7-dehydrocholesterol D 7 -reductase (DHCR7). We diagnosed SLOS in a fetus following intrauterine demise at 32 weeks' gestation. Chorionic villus (CV) sampling had been performed at 30 weeks because oligohydramnios and atrioventricular septal defect were noted on fetal ultrasound. On fetal post-mortem examination, a midline U-shaped soft palate cleft, micrognathia, postaxial polydactyly of the ®ngers with single transverse palmar creases bilaterally, and cutaneous syndactyly of toes two±three bilaterally suggested SLOS. We hypothesized that SLOS could be con®rmed by analysis of tissue sterols despite extensive autolysis, and by measurement of enzyme activity in CV cells. Measurement of DHCR7 activity in CV cells was undertaken using ergosterol as a substrate. CV cells were unable to convert any ergosterol to brassicasterol after a 72 h incubation period while control CV cells reduced 12.6±71.8% of ergosterol to brassciasterol in a 72 h period. SLOS was con®rmed by measurement of elevated 7-dehydrocholesterol (7-DHC) in the CV cells. Measurements of sterols were made in multiple fetal tissues. All tissues analysed showed elevated 7-DHC with markedly increased 7-DHC/cholesterol ratios.