๐”– Bobbio Scriptorium
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Estimation of glycogen in small amounts of tissue

โœ Scribed by Emile Van Handel

Publisher
Elsevier Science
Year
1965
Tongue
English
Weight
566 KB
Volume
11
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Most investigators

favor, for isolation of total glyeogen from animal tissues, the modification of Good et al. (1) of Pfluger's method (2), and assay the precipitate with the anthrone reagent (3). This combination of procedures yielded low and unreproducible results when applied to 0.1 mg of glycogen, to individual mosquitoes, or to 10 mg of liver. Therefore, each step of these procedures was reinvestigated.

By taking into consideration factors affecting the precipit.ability of aqueous glycogen solutions by ethanol, quantitative isolation of as little as 1 pg of glycogen from animal tissue was achieved.

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