Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis

✍ Scribed by Zheng Shen; Xiaojun Liu; Xiaomian Zhou; Aiye Liang; Dapeng Wu; Linfen Yu; Zhongpeng Dai; Jianhua Qin; Bingcheng Lin

• Book ID: 102925826
• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 592 KB
• Volume: 30
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200600530

✦ Synopsis

Abstract

Microfabricated capillary array electrophoresis (μ‐CAE) was applied to study the interaction between minor groove binder netropsin and a non‐selfcomplementary 12 mer double stranded oligodeoxynucleotide: d(CCCCTATACCGC)·d(GCGGTATAGGGG). ESI‐MS was used to provide an independent verification of the microchip electrophoresis derived data. Simultaneous parallel quantitative assay of multiple samples was performed in a single run (<50 s) on the self‐developed μ‐CAE device. The binding constant and stoichiometry calculated from Scatchard plot were (2.88 ± 0.23)×10^5^ M^–1^ and 1:1, respectively. The values showed a good quantitative agreement with the results determined by ESI‐MS and those using other methods reported in the literature.