Quantitative investigation of the interaction between granulocyte-macrophage colony-stimulating factor and heparin by capillary zone electrophoresis

✍ Scribed by Aiye Liang; Yuguang Du; Keyi Wang; Bingcheng Lin

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 367 KB
• Volume: 29
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200600084

✦ Synopsis

Quantitative investigation of the interaction between granulocyte-macrophage colonystimulating factor and heparin by capillary zone electrophoresis

The interactions between granulocyte-macrophage colony-stimulating factor (GM-CSF) and heparin or low-molecular weight heparin (LMWH) were studied by CZE. It was found that GM-CSF could bind to both heparin and LMWH. The binding constants were calculated from Scatchard regression to be (6.5 l 0.8)610 5 /M and (11.2 l 0.7)610 5 /M, respectively. The specificity of the interaction between GM-CSF and heparin was also studied by employing another sulfated j carrageenan oligosaccharide as a control. Results showed that j carrageenan oligosaccharide could not interact with GM-CSF, indicating that GM-CSF could specifically interact with heparin.