𝔖 Bobbio Scriptorium
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Quantitative evaluation of metal ion binding toPvuII restriction endonuclease

✍ Scribed by Thomas J. José; Lori H. Conlan; C. M. Dupureur

Book ID
106233015
Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
244 KB
Volume
4
Category
Article
ISSN
1432-1327

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✍ Mones, Letif; Simon, István; Fuxreiter, Monika 📂 Article 📅 2007 🏛 Walter de Gruyter GmbH & Co. KG 🌐 English ⚖ 142 KB

The number of metal ions required for phosphoryl transfer in restriction endonucleases is still an unresolved question in molecular biology. The two Ca 2q and Mn 2q ions observed in the pre-and post-reactive complexes of BamHI conform to the classical two-metal ion choreography. We probed the Mg 2q