𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Quantitative determination of titers of anti-zona serum

✍ Scribed by Tsunoda, Y. ;Sugie, T. ;Mori, J.

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
401 KB
Volume
207
Category
Article
ISSN
0022-104X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The titers of rabbit antiserum against isolated mouse zonae pellucidae were examined by several methods in connection with inhibitory effect on fertilization.

The titers determined by zona precipitate, zona dissolution, indirect immunofluorescence and in vitro fertilization tests were 2^4^, 2^1^ to 2^4^, 2^7^ and 2^4^, respectively. Cytotoxic effect could not be detected from zona antibody. The indirect immunofluorescence was most sensitive for detection of zona antibody but did not represent the extent of inhibitory effect on fertilization. The titer obtained by zona precipitate test was most close to the titer obtained by inhibitory effect on in vitro fertilization.

The present study also demonstrated that at least 0.0375 ml of antiserum per female mouse, equivalent to 0.15–0.25% of body weight, was necessary for inhibition of fertilization in vivo by passive immunization with anti‐zona serum.

πŸ“œ SIMILAR VOLUMES

Quantitative determination of fenclorac
Quantitative determination of fenclorac in serum
✍ J. K. Lee; R. D. Smyth; A. Polk; T. Herczeg; C. T. Tsuei; N. H. Reavey-Cantwell πŸ“‚ Article πŸ“… 1977 πŸ› John Wiley and Sons 🌐 English βš– 334 KB

A spectrophotometric method for the analysis of fenclorac and its metabolite, 3-chloro-4-cyclohexylbenzeneglycolic acid, in human serum was developed. The parent compound represented at least 90% of the total species present in blood; the metabolite was present to the extent of about 10%, primarily

Immunological specificity of anti-zona b
Immunological specificity of anti-zona binding to zona pellucida
✍ Sacco, Anthony G. πŸ“‚ Article πŸ“… 1978 πŸ› John Wiley and Sons 🌐 English βš– 343 KB

## Abstract Biochemically purified and F(abβ€²)~2~ immunoglobulin fractions were prepared from several antisera, including a human serum, which contained anti‐zona pellucida activity. Antizona activity was monitored either by the formation of the antibody produced precipitation layer on the zona foll

Quantitative automated latex nephelometr
Quantitative automated latex nephelometric immunoassay for determination of myoglobin in human serum
✍ Luis Borque; Antonio Rus; Roberto Ruiz πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 415 KB

We have evaluated a new latex nephelometric test for the quantitation of myoglobin in human serum. The assay consists of incubating the diluted serum sample (20-fold) for 12 min at room temperature with latex particles covalently coated with anti-myoglobin antibodies and then quantifying the change