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Immunological specificity of anti-zona binding to zona pellucida

✍ Scribed by Sacco, Anthony G.

Publisher: John Wiley and Sons
Year: 1978
Tongue: English
Weight: 343 KB
Volume: 204
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402040206

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✦ Synopsis

Abstract

Biochemically purified and F(ab′)~2~ immunoglobulin fractions were prepared from several antisera, including a human serum, which contained anti‐zona pellucida activity. Antizona activity was monitored either by the formation of the antibody produced precipitation layer on the zona following antiserum treatment and/or by the indirect fluorescent antibody method. No changes in anti‐zona activity were observed when the purified and the digested immunoglobulin preparations from each antiserum were compared to their whole serum counterparts. It is concluded that anti‐zona binding to the zona as well as the formation of the precipitation layer on the zona is the result of a specific antigen antibody reaction.

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