Quantitative determination of paraffin oil in blood by capillary gas chromatography

✍ Scribed by Tsikas, Dimitrios ;Brunner, Georig

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 304 KB
• Volume: 13
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240130406

✦ Synopsis

Abstract

A capillary gas chromatographic method is described for the quantitative determination of liquid paraffin in blood. Paraffin is extracted from blood into n‐heptane. After solvent evaporation and dissolution of the residue in 100–200 μl n‐heptane one μl is injected into a gas chromatograph fitted with a fused silica capillary column (Permabond® OV‐1‐CB‐0.1, 10 m × 0.32 mm i.d.) and flame ionization detector. Analysis is performed by using an oven program [50°C (3 min)−285°C (5 min), rise 10%min]. The sensitivity (1.5 ng hexadecane) and the reproducibility prove the applicability of the method for the determination of liquid paraffin in blood and for the study of the stability of the liquid paraffin hollow fiber membranes used in an extracorporeal liver support system.