✦ LIBER ✦

Quantitative analysis of polymerase chain reaction products with high performance liquid chromatography and fluorometric detection

✍ Scribed by A. Chan; Z. Jui; M. Krajden; E.P. Diamandis

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 113 KB
Volume: 26
Category: Article
ISSN: 0009-9120
DOI: 10.1016/0009-9120(93)90074-g

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✦ Synopsis

conditions, but there existed a linear relationship between the molar ratio of input targets and the molar ratio of their corresponding output products. Using this mathematical approach, we have achieved very good accuracy (recovery %) of 100 + 16% and within-run imprecision (CV) of 12% for a quantitative PCR assay of input native CMV AD169 DNA target sequence over a 5 log range (0.0031 to 31 amoles, corresponding to 2 x 103 -2 x 107 copies of DNA target sequence). Improvement in sample preparation and optimization of primer-target sequences are necessary before this methodology can be applied clinically. This quantitative PCR detection method provides an analytical model to refine PCR-based techniques.

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