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Quantitative analysis of polymerase chain reaction (PCR) products using primers labeled with biotin and a fluorescent dye

✍ Scribed by Axel Landgraf; Bernd Reckmann; Alfred Pingoud

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
849 KB
Volume
193
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

PCR primers covalently labeled with biotin and a fluorescent dye allow immobilization and separation of the products which can be quantitatively analyzed subsequently. The procedure we have developed circumvents electrophoretic separation and radioactive labeling. Exact quantitative analysis of reaction products is feasible during the logarithmic phase of amplification when Taq polymerase is not limiting, as it is during the plateau phase of the reaction. With appropriate standardization the procedure can be used for routine diagnostic purposes.

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