A method to determine the proportions of the major fiber-forming collagens (types I, III, and V) in noncartilaginous human tissues is presented. The procedure relies on direct solubilization of tissue collagen as cyanogen bromide peptides. The peptides are subjected to cation exchange chromatography
Quantitation of type I and III collagens using electrophoresis of alpha chains and cyanogen bromide peptides
โ Scribed by Danny Chan; William G. Cole
- Book ID
- 107711967
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 575 KB
- Volume
- 139
- Category
- Article
- ISSN
- 0003-2697
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Fetal calf skin was extracted with neutral buffer in the presence of enzyme inhibitors. Type III collagen was isolated from the extract and cleaved with cyanogen bromide. The N-and C-terminal cyanogen bromide peptides were purified and characterized. The sizes and amino acid compositions of the N-an