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Periodic acid-Schiff staining of type II collagen and its cyanogen bromide peptides after polyacrylamide gel electrophoresis

✍ Scribed by Dr. Jess C. Rajan; Leroy Klein


Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
398 KB
Volume
3
Category
Article
ISSN
0173-0835

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✦ Synopsis


Abstract

Pepsin‐solubilized type II collagen and some of its CNBr‐derived peptides could be stained for carbohydrate with periodic acid‐Schiff (PAS) after polyacrylamide gel electrophoresis. PAS intensely stained an aldehyde‐containing peptide (M~r~ > 10 000) which was not detected with Coomassie Brilliant Blue R‐250 stain. Types I and III collagens and their CNBr peptides stained poorly, if at all, with PAS.