Purification and characterization of the N- and C-terminal cyanogen bromide peptide of salt-soluble type III collagen

✍ Scribed by R.W. Glanville; P.P. Fietzek

• Book ID: 102628112
• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 396 KB
• Volume: 80
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90646-7

✦ Synopsis

Fetal calf skin was extracted with neutral buffer in the presence of enzyme inhibitors. Type III collagen was isolated from the extract and cleaved with cyanogen bromide. The N-and C-terminal cyanogen bromide peptides were purified and characterized. The sizes and amino acid compositions of the N-and C-terminal non-triple-helical extensions of type III collagen were deduced.