Nucleotides, nucleosides, and purine bases were extracted from human endomyocardial biopsies, freeze-clamped rat hearts, and porcine coronary sinus plasma. Perchloric acid extracts were neutralized with Freon-trioctylamine and analyzed at 250 nm by reverse-phase ion-pairing high-performance liquid c
Quantitation of nucleotides, nucleosides and bases in antemortem and postmortem bloodstains by high-performance liquid chromatography
β Scribed by H. Sugie; T. Nishikawa; T. Funao
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 419 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0379-0738
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A single-column high-performance liquid chromatographic system for the simultaneous separation of bases, nucleosides and nucleoside mono-and polyphosphates has been developed, in which a strong porous anion-exchange resin (Aminex A-14) is used. The chromatographic run, carried out at 55" and at alka
A high-performance liquid chromatography (HPLC) method is described for the separation and quantitation of nucleotides, nucleosides, purine bases, and related compounds in one single run. The separation of a standard mixture of at least 24 components is achieved within 35 min on glass columns (30 cm