Quantitation of mRNA and protein products of the Ha-ras-1 proto-oncogene
β Scribed by Roberto Gambari; Patrizio Giacomini
- Book ID
- 105315308
- Publisher
- Springer US
- Year
- 1990
- Tongue
- English
- Weight
- 175 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0167-6806
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Two studies on breast cancer patients are described. Our aim was to examine whether the combined frequency of rare c-Ha-ras-l alleles in cancer patients was raised. Firstly, the c-Ha-ras-l locus in 56 breast cancer patients and 48 controls was examined for restriction fragment length polymorphism (R
Activation of the Ha-ras proto-oncogene, but not the Ki-ras or N-ras genes, has been found in mammary gland carcinomas induced in female rats by a single dose of methylnitrosourea (MNU). Here we show that a 10-kb restriction fragment containing the Ha-ras gene was extensively methylated by MNU in DN
## Abstract The mutagenicity of oxygen free radicals was studied in a forward mutation system. pEC plasmid containing the human cβHaβ__ras__β1 protoβoncogene was reacted with oxygen free radicals generated by Cu^2+^ and H~2~O~2~ and was then transfected into NIH/3T3 cells. Transformed foci were obs