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Quantitation of enzymatically generated hydrogen peroxide based on aqueous peroxyoxalate chemiluminescence

✍ Scribed by M.L. Grayeski; E.J. Woolf; P.J. Helly

Publisher: Elsevier Science
Year: 1986
Tongue: English
Weight: 635 KB
Volume: 183
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(86)80088-5

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✦ Synopsis

The method involves the reaction of 4,4'-{oxalyl bis[(trifluoromethylsulfonyl)imino]-ethylene}bis(4-methylmorpholinium trifluoromethanesulfonate) with hydrogen peroxide in the presence of rhodamine-B. Precise measurements, with l-3% relative standard deviation, can be made in both static and flow systems. In the flow system, the response to hydrogen peroxide is linear from 10 -* M hydrogen peroxide down to the limit of detection of 7 x lo-' M.

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