Detection of substances with alcoholic or phenolic hydroxyl groups by generation of hydrogen peroxide with imidazole and peroxyoxalate chemiluminescence

✍ Scribed by Nozaki, Osamu ;Iwaeda, Toshinao ;Kato, Yoshio

• Publisher: John Wiley and Sons
• Year: 1995
• Weight: 444 KB
• Volume: 10
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170100606

✦ Synopsis

On-line detection o f substances with an alcoholic or phenolic hydroxyl group using imidazole and peroxyoxalate chemiluminescence was investigated qualitatively using a flow-injection method. The substances tested included six polyphenols, five monophenols and six sugars. After incubation at 80°C w i t h an imidazole buffer (pH 9.5) the substances were detected by peroxyoxalate chemiluminescence. The polyphenols tested (e.g., pyrogallol. purpurogallin, and dopamine) showed the strongest light emission. The sugars with hydroxyl groups (e.g.. fructose and lactose) and the monophenols (e.g., phenol, serotonin, and 0-estradiol) produced only a weak light emission. Reaction of hydroxyl compounds and imidazole generated hydrogen peroxide. lmidazole served t w o roles, it catalysed the reaction w i t h the hydroxyl compound and initiated peroxyoxalate chemiluminescence on-line. A novel reactor formed by packing glass beads into a f l o w cell (Teflon) of a chemiluminometer improved the sensitivity o f light detection.