Highly sensitive detection of L-lactate and pyruvate by amperometric flow-injection analysis based on enzymatic substrate recycling and selective detection of hydrogen peroxide

Highly sensitive determination of L-lact

Highly sensitive determination of L-lactate and pyruvate by liquid chromatography and amperometric detection with lactate oxidase-lactate dehydrogenase coimmobilized reactor involving amplification by substrate recycling

✍ Toshio Yao; Naokazu Kobayashi; Tamotsu Wasa 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 439 KB

An amperometric flow injection system with a lactate oxidase-lactate dehydrogenase coimmobilized reactor involving amplification by substrate recycling is used as a specific postcolumn detector system in liquid chromatography, to detect L-lactate and pyruvate with high sensitivity. Both components a