Quantitation of antioxidants in water samples using ionic liquid dispersive liquid–liquid microextraction followed by high-performance liquid chromatography-ultraviolet detection

✍ Scribed by Hamid Reza Sobhi; Amir Kashtiaray; Hadi Farahani; Mohammad Reza Farahani

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 176 KB
• Volume: 34
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201000526

✦ Synopsis

Abstract

A simple and efficient method, ionic liquid‐based dispersive liquid–liquid microextraction combined with high‐performance liquid chromatography‐ultraviolet detection (HPLC‐UV), has been applied for the extraction and determination of some antioxidants (Irganox 1010, Irganox 1076 and Irgafos 168) in water samples. The microextraction efficiency factors were investigated and optimized: 1‐hexyl‐3‐methylimidazolium hexafluorophosphate [C~6~MIM][PF~6~] (0.06 g) as extracting solvent, methanol (0.5 mL) as disperser solvent without salt addition. Under the selected conditions, enrichment factors up to 48‐fold, limits of detection (LODs) of 5.0–10.0 ng/mL and dynamic linear ranges of 25–1500 ng/mL were obtained. A reasonable repeatability (RSD≤11.8%, n=5) with satisfactory linearity (r^2^≥0.9954) of the results illustrated a good performance of the presented method. The accuracy of the method was tested by the relative recovery experiments on spiked samples, with results ranging from 85 to 118%. Finally, the method was successfully applied for determination of the analytes in several real water samples.