๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Quantifying Ligand Binding to Large Protein Complexes Using Electrospray Ionization Mass Spectrometry

โœ Scribed by El-Hawiet, Amr; Kitova, Elena N.; Arutyunov, Denis; Simpson, David J.; Szymanski, Christine M.; Klassen, John S.


Book ID
118154125
Publisher
American Chemical Society
Year
2012
Tongue
English
Weight
602 KB
Volume
84
Category
Article
ISSN
0003-2700

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


Observation of large subunit protein com
โœ Joseph A. Loo ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 431 KB

## Abstract Mass spectrometry with electrospray ionization and a magnetic sector instrument was used to detect multiply charged molecules for the nonโ€covalently bound dimeric subunit protein complexes of horse liver alcohol dehydrogenase (__M__~r~ โˆผ 80 000) and the tetrameric complexes of yeast alc

Binding constant determination of high-a
โœ Arno Wortmann; Matthias C. Jecklin; David Touboul; Martin Badertscher; Renato Ze ๐Ÿ“‚ Article ๐Ÿ“… 2008 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 600 KB

## Abstract We describe an approach for the determination of binding constants for proteinโ€“ligand complexes with electrospray ionization mass spectrometry, based on the observation of unbound ligands competing for binding to a protein target. For the first time, dissociation constants lower than pi

Investigation of non-covalent ligand bin
โœ Brenda L. Schwartz; David C. Gale; Richard D. Smith; Ashutosh Chilkoti; Patrick ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 801 KB

## Abstract The relative nonโ€covalent binding behavior of two small molecule ligands to the tetrameric protein streptavidin was investigated by electrospray ionization mass spectrometry (ESIโ€MS). An extended __m__/__z__ range quadrupole mass spectrometer was employed to observe the intact multimeri