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Observation of large subunit protein complexes by electrospray ionization mass spectrometry

✍ Scribed by Joseph A. Loo

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
431 KB
Volume
30
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

Mass spectrometry with electrospray ionization and a magnetic sector instrument was used to detect multiply charged molecules for the non‐covalently bound dimeric subunit protein complexes of horse liver alcohol dehydrogenase (M~r~ ∼ 80 000) and the tetrameric complexes of yeast alcohol dehydrogenase (M~r~ ∼ 147 000) and rabbit muscle pyruvate kinase (M~r~ ∼ 232 000). Ions for the pyruvate kinase complex represent one of the largest intact protein complexes resolved by mass spectrometry. Solvation of the large gas phase complexes is indicated by the mass spectrometric results.

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