Root segments of Pisum sativum L. were transformed by several strains of Agrobacterium rhizogenes. The resulting hairy roots, as well as apical segments from untransformed pea roots, were used to initiate root lines cultured in vitro. Levels of free IAA were quantified in the sub-cultured lines by g
Quantification of indol-3-yl acetic acid in pea and maize seedlings by gas chromatography-mass spectrometry
β Scribed by James R.F. Allen; Laurent Rivier; Paul-Emile Pilet
- Book ID
- 107842393
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 646 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0031-9422
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Indole-3-acetic acid (IAA) was identified and quantified in three cultured tumour lines, which included crown-gall tissue of Datura (25.4 rig/g fresh wt.) and two genetic tumour lines of tobacco (4.6 and 8.0ng/g fresh wt. ). The analysis was carried out using a stable isotope dilution assay in comb
Seven day old seedlings of Pisum sativum L., cv. Kleine RheinlΓ€nderin, were wilted for 3 days. After partially removing the roots, they were rewatered and at the same time radioactive abscisic acid([1-(14)C]ABA, spec. activity 1.7Β·10(8)d s(-1)mmol(-1)) was applied for 1 h via the xylem of the roots.