Indole-3-acetic acid determination in cultured crown-gall and genetic tumour tissues by gas chromatography-mass spectrometry

✍ Scribed by L. M. S. Palni; R. E. Summons

• Book ID: 104616505
• Publisher: Springer Netherlands
• Year: 1987
• Tongue: English
• Weight: 506 KB
• Volume: 5
• Category: Article
• ISSN: 0167-6903
• DOI: 10.1007/bf00035019

✦ Synopsis

Indole-3-acetic acid (IAA) was identified and quantified in three cultured tumour lines, which included crown-gall tissue of Datura (25.4 rig/g fresh wt.) and two genetic tumour lines of tobacco (4.6 and 8.0ng/g fresh wt.

). The analysis was carried out using a stable isotope dilution assay in combination with gas chromatography-mass spectrometry. This is the first unambiguous determination of endogenous IAA in genetic tumour tissues.