Quantification of hepatitis B virus DNA using competitive PCR and a scintillation proximity assay
β Scribed by P.R Simpson; X.H Yu; Z.M Redza; J.G Anson; S.H Chan; Y Lin
- Book ID
- 114140468
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 193 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0166-0934
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DNA gyrase is the target of coumarin and cyclothialidine antibacterials, which bind to the B subunit of the enzyme (GyrB). Currently available GyrB inhibitors have not been clinically successful, but their high in vitro potency against DNA gyrase has raised interest in the development of novel nonco
## Abstract The application of a new fluorogenic probeβbased PCR assay (PCR duplex scorpion primer assay) to the detection of Hepatitis B virus (HBV) DNA in human sera was described. Duplex scorpion primer is a modified variant of duplex Amplifluor, and the incorporation of a PCR stopper between pr