✦ LIBER ✦

Detection of hepatitis B virus DNA by duplex scorpion primer-based PCR Assay

✍ Scribed by De-Ming Kong; Han-Xi Shen; Huai-Feng Mi

Publisher: John Wiley and Sons
Year: 2010
Tongue: English
Weight: 447 KB
Volume: 22
Category: Article
ISSN: 0256-7660
DOI: 10.1002/cjoc.20040220906

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✦ Synopsis

Abstract

The application of a new fluorogenic probe‐based PCR assay (PCR duplex scorpion primer assay) to the detection of Hepatitis B virus (HBV) DNA in human sera was described. Duplex scorpion primer is a modified variant of duplex Amplifluor, and the incorporation of a PCR stopper between probe and primer sequences improve the detection specificity and sensitivity. Combined with PCR amplification, this probe can give unambiguous positive results for the reactions initiated with more than 20 HBV molecules. In addition, the particular unimolecular probing mechanism of this probe makes the use of short target‐specific probe sequence possible, which will render this probe applicable in some specific systems.

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