Label-free DNA sensor based on fluorescent cationic polythiophene for the sensitive detection of hepatitis B virus oligonucleotides

✍ Scribed by Hongliang Guan; Min Cai; Lu Chen; Yun Wang; Zhike He

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 475 KB
• Volume: 25
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.1151

✦ Synopsis

Abstract

Water‐soluble fluorescent conjugated polymers can be used as an optical platform in highly sensitive DNA sensors. Here we report a simple label‐free DNA sensor using poly(3‐alkoxy‐4‐methylthiophene) to recognize and detect different oligonucleotide targets related to the YMDD gene mutation of hepatitis B virus. The concentration of surfactant Triton X‐100, NaCl, the oligonucleotide capture probe and the oligonucleotide hybridization conditions have a great impact on fluorescence intensity. Under the optimum conditions, two types of oligonucleotide targets involving YMDD gene mutation of hepatitis B virus were successfully recognized. Moreover, there was a linear relationship between fluorescence intensity and the concentration of oligonucleotide target. The detection limit of the wild‐type hepatitis B virus target is 88 pmol L^−1^. Copyright © 2009 John Wiley & Sons, Ltd.