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Quantification of cells in islets of langerhans using DNA determination

✍ Scribed by Beckmann, Jürgen ;Lenzen, Sigurd ;Holze, Susanne ;Panten, Uwe

Publisher
Springer-Verlag
Year
1981
Weight
412 KB
Volume
18
Category
Article
ISSN
0001-5563

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A method is described determining the pyruvate content in l-10 pg of lyophilized tissue. This represents a range of weight much below that of fine-needle biopsies. NAD+, generated through the enzymatic conversion of pyruvate to lactate, is reduced to NADH which is then assayed by its ability to indu