Analysis of oligonucleotides with gel-filled capillary columns is a fast, efficient and automated way to check their purity. Gel-filled columns were optimized to separate oligonucleotides which were 2&50 nucleotides in length. The influence of sample size on resolution is discussed. A comparison bet
Qualitative and Quantitative Determination of Oligonucleotides by Non-Gel Capillary Electrophoresis
β Scribed by Wujie Li; Yinling Ma; Zhu Gan; Xiaomei Ling; Zhenjun Yang
- Book ID
- 106317046
- Publisher
- Springer
- Year
- 2011
- Tongue
- English
- Weight
- 214 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0009-5893
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## Abstract Diffusion coefficients of oligonucleotides have been measured in polyacrylamide gel capillaries without application of an electrical field. A relationship between such diffusion coefficients and molecular size of the oligonucleotides could be achieved. The slower diffusion of larger oli
Plasmids may appear in different forms: circular with different degrees of coiling, partially cleaved or linear, and multimeric as concatamers or catenates. Capillary gel electrophoresis (CGE) of plasmid samples allows the determination of plasmid form distribution. Monomeric and dimeric plasmid DNA