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Qualitative and Quantitative Determination of Oligonucleotides by Non-Gel Capillary Electrophoresis

✍ Scribed by Wujie Li; Yinling Ma; Zhu Gan; Xiaomei Ling; Zhenjun Yang


Book ID
106317046
Publisher
Springer
Year
2011
Tongue
English
Weight
214 KB
Volume
73
Category
Article
ISSN
0009-5893

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Analysis of oligonucleotides with gel-filled capillary columns is a fast, efficient and automated way to check their purity. Gel-filled columns were optimized to separate oligonucleotides which were 2&50 nucleotides in length. The influence of sample size on resolution is discussed. A comparison bet

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## Abstract Diffusion coefficients of oligonucleotides have been measured in polyacrylamide gel capillaries without application of an electrical field. A relationship between such diffusion coefficients and molecular size of the oligonucleotides could be achieved. The slower diffusion of larger oli

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