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Analysis of oligonucleotides by capillary gel electrophoresis

✍ Scribed by Aran Paulus; J.I. Ohms

Book ID
104145609
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
744 KB
Volume
507
Category
Article
ISSN
1873-3778

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✦ Synopsis

Analysis of oligonucleotides with gel-filled capillary columns is a fast, efficient and automated way to check their purity. Gel-filled columns were optimized to separate oligonucleotides which were 2&50 nucleotides in length. The influence of sample size on resolution is discussed. A comparison between capillary gel electrophoresis and slab gel electrophoresis in terms of analysis time per sample is made. In addition to homopolymeric samples, the separation of heteropolymeric oligonucleotides with one nucleotide difference in size is presented and suggests the possibility of using capillary gel electrophoresis as a tool for DNA sequencing.

πŸ“œ SIMILAR VOLUMES

Diffusion coefficients of oligonucleotid
Diffusion coefficients of oligonucleotides in capillary gel electrophoresis
✍ Hong-Feng Yin; Maria Helen Kleemiß; JΓΌrgen Alfred Lux; Gerhard Schomburg πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 406 KB

## Abstract Diffusion coefficients of oligonucleotides have been measured in polyacrylamide gel capillaries without application of an electrical field. A relationship between such diffusion coefficients and molecular size of the oligonucleotides could be achieved. The slower diffusion of larger oli