𝔖 Bobbio Scriptorium
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Qualitative analysis of indigo precursors from woad by HPLC and HPLC-MS

✍ Scribed by K. G. Gilbert (nee Stoker); D. J. Hill; C. Crespo; A. Mas; M. Lewis; B. Rudolph; D. T. Cooke

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
46 KB
Volume
11
Category
Article
ISSN
0958-0344

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✦ Synopsis

A qualitative method has been devised to analyse the indigo precursors in leaf extracts of woad (Isatis spp.) using HPLC coupled to an evaporative light scattering detector and confirmed using HPLC coupled, on line, to a particle beam mass spectrometer. Using this technique, indican has been positively identified in shoot extracts of European (I. tinctoria) and Chinese (I. indigotica) woad. Greater amounts of indican were found in the Chinese compared with the European woad.

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## Abstract A novel method, HPLC‐MS/MS was developed to qualitatively identify and quantitatively determine the 28 components including 19 diterpenoids, 6 phenolic acids and 3 flavonoids in __Isodon rubescens__, an important traditional Chinese medicine. The separation was performed on a C~18~ colu