A modified C-banding technique is described that produces C bands on human chromosomes after sequential Q and R banding and retains good chromosome morphology. Despite the considerable exposure to UV light during sequential Q and R bandings, clear C bands could still be achieved. Employing the prese
Q-banding of human chromosomes after BUdR and BCdR treatment
✍ Scribed by Erica M. Bühler; Taiso Tsuchimoto; György Kosztolànyi; L. Pia Jurik
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 498 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0340-6717
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✦ Synopsis
Two different Q patterns were found in BUdR and BCdR treated chromosomes of human lymphocyte cultures: X-type pattern, in which Giemsa and quinacrine banding both are reversed; Y-type pattern, in which Q-banding remains conventional in spite of reverse G-banding. Possible mechanisms of these findings are discussed.
📜 SIMILAR VOLUMES
A modified thermal denaturation of human chromosomes in Hanks' solution at 91 degrees C produces highly contrasted C and T bands. This improved double-banding system is a good tool in the analysis of ring chromosomes, for which R banding is often inadequate, as it is exemplified.