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Production of C and T bands in human mitotic chromosomes after heat treatment

✍ Scribed by Yves Chamla; Marie Ruffié

Publisher
Springer
Year
1976
Tongue
English
Weight
504 KB
Volume
34
Category
Article
ISSN
0340-6717

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✦ Synopsis

A modified thermal denaturation of human chromosomes in Hanks' solution at 91 degrees C produces highly contrasted C and T bands. This improved double-banding system is a good tool in the analysis of ring chromosomes, for which R banding is often inadequate, as it is exemplified.

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Q-banding of human chromosomes after BUd
Q-banding of human chromosomes after BUdR and BCdR treatment
✍ Erica M. Bühler; Taiso Tsuchimoto; György Kosztolànyi; L. Pia Jurik 📂 Article 📅 1976 🏛 Springer 🌐 English ⚖ 498 KB

Two different Q patterns were found in BUdR and BCdR treated chromosomes of human lymphocyte cultures: X-type pattern, in which Giemsa and quinacrine banding both are reversed; Y-type pattern, in which Q-banding remains conventional in spite of reverse G-banding. Possible mechanisms of these finding