Banding of unfixed mitotic chromosomes in suspension after release from human lymphocytes and fibroblasts

✍ Scribed by Charles H. C. M. Buys; Trijntje Koerts; Ameke Y. Veen

Publisher: Springer
Year: 1984
Tongue: English
Weight: 674 KB
Volume: 66
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00287642

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Production of C and T bands in human mitotic chromosomes after heat treatment

✍ Yves Chamla; Marie Ruffié 📂 Article 📅 1976 🏛 Springer 🌐 English ⚖ 504 KB

A modified thermal denaturation of human chromosomes in Hanks' solution at 91 degrees C produces highly contrasted C and T bands. This improved double-banding system is a good tool in the analysis of ring chromosomes, for which R banding is often inadequate, as it is exemplified.