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Purine mutants of mammalian cell lines: III control of purine biosynthesis in adenine phosphoribosyl transferase mutants of CHO cells

โœ Scribed by M. W. Taylor; J. H. Pipkorn; M. K. Tokito; R. O. Pozzatti


Book ID
118290171
Publisher
Springer
Year
1977
Tongue
English
Weight
551 KB
Volume
3
Category
Article
ISSN
1572-9931

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A class of purine auxotrophs blocked early in the purine biosynthetic pathway was examined. The inability of these mutants to accumulate formylglycinamide ribotide (FGAR) in the presence of azaserine suggested that one or more of the first three enzymes of the pathway were either missing or defectiv

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## Abstract When added to medium containing coformycin (2 ฮผM or above), adenine is lethal to Chinese hamster fibroblasts at the concentration inhibiting de novo purine biosynthesis (Debatisse and Buttin, '77b). Rescue by hypoxanthine suggested that cells die of IMP starvation when the analog can tu