Four new symmetrical colourless biomimetics bearing two monochlorotriazine rings have been rationally designed and synthesized. These immobilized colourless ligands and one analogue of Cibacron Blue F3GA on Sepharose CL-4B were used to purify alcohol dehydrogenase from baker's yeast extract. Twenty-
Purification of yeast alcohol dehydrogenase by Congo red affinity chromatography
✍ Scribed by A.H. Schmidt; H. Wombacher
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 169 KB
- Volume
- 519
- Category
- Article
- ISSN
- 1873-3778
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Glucose 6-phosphate dehydrogenase (EC 1.1.1.39) has been purified to homogeneity from baker's yeast by a simple procedure involving affinity elution from a column of red triazine dye, H-8BN, immobilized to Sepharose 6B. Eight milligrams of homogeneous protein is obtained in 53% yield from 200 g of d
## Abstract Das konventionelle Reinigungsverfahren von Glucose‐6‐phosphat‐dehydrogenase (G6PDH) (E.C. 1.1.1.49) aus Rinder‐Nebennierenrinde wird durch eine einzige affinitätschromatographische Trennungsstufe ersetzt. Die Anwendung einer mit Nicotinamid‐Adenin‐Dinukleotid‐Phosphat (NADP) substituier