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Purification of spin-labelled DNA by hydroxyapatite chromatography

✍ Scribed by I. Ivanov; E. Raikova; Z. Zaikov; D. Kaffalieva; J. Yaneva

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
319 KB
Volume
260
Category
Article
ISSN
1873-3778

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When 0.48 M NaPB is applied, the samples should be diluted to 0.1 M NaPB before loading. For NaCl, the concentration should not be lower than 2 M. When using alkaline reagents (NaCN, Na$), the solutions should be buffered to prevent denaturation of DNA (see footnote to Table 2).