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Alkylating spin labels for spin-labelling of DNA

โœ Scribed by I. Ivanov; E. Raikova; Z. Raikov; J. Yaneva; D. Kaffalieva


Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
436 KB
Volume
15
Category
Article
ISSN
0020-711X

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A spin-label assay for lysozyme, which is based on the enzymatic hydrolysis of spin-labeled peptidoglycan, is described. Hydrolysis of this polymer by lysozyme results in sharpening of the esr spectrum. The rate of spectral sharpening is a function of enzyme concentration. When the activities of hen