๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Spin-label assay for lysozyme

โœ Scribed by Laurance S. Johnston; Francis C. Neuhaus

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
393 KB
Volume
85
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

A spin-label assay for lysozyme, which is based on the enzymatic hydrolysis of spin-labeled peptidoglycan, is described. Hydrolysis of this polymer by lysozyme results in sharpening of the esr spectrum. The rate of spectral sharpening is a function of enzyme concentration. When the activities of hen egg-white and human lysozymes are compared by this method, human lysozyme is 3.5 times as active as the hen enzyme. The pH optima for both enzymes are pH 5.0. At this pH, the maximal activity for the hen egg-white lysozyme is observed at an ionic strength of 0.09. This assay is suitable for measuring lysozyme levels in biological fluids. It is a sensitive, continuous assay that measures muramidase activity on a defined substrate.

๐Ÿ“œ SIMILAR VOLUMES

Spin-label assay for phospholipase A2
Spin-label assay for phospholipase A2
โœ Ching-San Lai; Jian-Zhong Zhang; Joy Joseph ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 483 KB

A rapid and continuous method for measuring phospholipase A2 activity using electron spin resonance spectroscopy and a spin-labeled phospholipid as a substrate has been developed. The substrate, 1-palmitoyl-2-(4-doxylpentanoyl)glycerophosphocholine, gives rise principally to a broad ESR line in aque

A spin-reduction assay for lipoxidase
A spin-reduction assay for lipoxidase
โœ Wallace Snipes; Kathryn Kenny; Alec Keith ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 374 KB

Spin-label signals are reduced when the nitroxide is present in a lipoxidase reaction mixture. This spin reduction can be used as an assay for enzyme activity in turbid systems where the conventional uv assay cannot be used. Data taken with the spin-reduction assay show that phospholipid preparatio

Spin-Labeled Fulleropyrrolidines
Spin-Labeled Fulleropyrrolidines
โœ Carlo Corvaja; Fosca Conti; Lorenzo Franco; Michele Maggini ๐Ÿ“‚ Article ๐Ÿ“… 2007 ๐Ÿ› John Wiley and Sons โš– 11 KB

## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.