✦ LIBER ✦

A spin-reduction assay for lipoxidase

✍ Scribed by Wallace Snipes; Kathryn Kenny; Alec Keith

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 374 KB
Volume: 81
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90713-8

No coin nor oath required. For personal study only.

✦ Synopsis

Spin-label signals are reduced when the nitroxide is present in a lipoxidase reaction mixture.

This spin reduction can be used as an assay for enzyme activity in turbid systems where the conventional uv assay cannot be used. Data taken with the spin-reduction assay show that phospholipid preparations containing cholesterol are more resistant to oxidation by lipoxidase than are cholesterol-free preparations.

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