✦ LIBER ✦

Reduction of N-acetyl methionine sulfoxide: A simple assay for peptide methionine sulfoxide reductase

✍ Scribed by Nathan Brot; Jean Werth; Daryl Koster; Herbert Weissbach

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 329 KB
Volume: 122
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90283-4

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✦ Synopsis

A simple and rapid assay has been developed to measure the enzymatic activity of peptide methionine sulfoxide reductase. The assay is based on the reduction of labeled N-acetylmethionine sulfoxide to N-acetvlmethionine. The N-acetylmethionine can be separated from the substrate by extraction into ethyl acetate.