Purification of rosmarinic acid synthase from cell cultures ofColeus blumeiBenth
β Scribed by Maike Petersen
- Publisher
- Springer-Verlag
- Year
- 1993
- Tongue
- English
- Weight
- 646 KB
- Volume
- 191
- Category
- Article
- ISSN
- 0032-0935
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π SIMILAR VOLUMES
In order to study the accumulation and transport of rosmarinic acid in suspension cells of Coleus blumei we established an efficient method to isolate protoplasts and vacuoles. Protoplasts were disrupted by an osmotic shock in a medium with basic pH containing ethylenediamine tetraacetic acid. The r
Phenylalanine ammonia-lyase from an "over-producer" strain of Coleus blurnei Benth. cell cultures accumulating high levels of rosmarinic acid (RA) has been shown to possess no special feed-back sensitivity to RA or its precursors. No tyrosine-3hydroxylase activity could be detected in culture extrac