Interactions between parallel pathways during biosynthesis of rosmarinic acid in cell suspension cultures ofColeus blumei
✍ Scribed by B. E. Ellis; S. Remmen; G. Goeree
- Publisher
- Springer-Verlag
- Year
- 1979
- Tongue
- English
- Weight
- 488 KB
- Volume
- 147
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
✦ Synopsis
Phenylalanine ammonia-lyase from an "over-producer" strain of Coleus blurnei Benth. cell cultures accumulating high levels of rosmarinic acid (RA) has been shown to possess no special feed-back sensitivity to RA or its precursors. No tyrosine-3hydroxylase activity could be detected in culture extracts and no specific inhibitors of tyrosine incorporation into RA were found. L-~-aminooxy-fi-phenyl propionic acid, however, was effective in specifically blocking phenylalanine incorporation. This block also led to an accumulation of label from tyrosine in 4hydroxyphenyllactic acid rather than in 3,4-dihydroxyphenylalanine (DOPA) or 3,4-dihydroxyphenyllactic acid. These observations require a re-evaluation of the possible role of DOPA as a major biogenic precursor to RA.