Purification of plastid DNA from tobacco cell suspensions
โ Scribed by Seyer, P.; Herrmann, R.G.; Lescure, A.-M.
- Book ID
- 121930472
- Publisher
- Elsevier Science
- Year
- 1982
- Weight
- 631 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0304-4211
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๐ SIMILAR VOLUMES
From isolated nuclei of suspension cultured cells of Nicotiana tabacum. DNA-dependent RNA polymerase II (E.C. 2.7.76) has been purified to homogeneity as evidenced by polyacrylamidegel electrophoresis under non-denaturing conditions. The purified enzyme had a specific activity of more than 15 nmol m
Mitochondrial DNA of tobacco supension culture cells labeled in vivo with a H-thymidine was isolated from purified mitochondria. It has a density of 1.707 + 0.002 g/cm 3 unique from both nuclear and chloroplast DNA. Approximately 2545% of the mtDNA molecules from several preparations from suspension