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Purification of mitochondrial and plastid DNA

โœ Scribed by Lang, B Franz; Burger, Gertraud


Book ID
109946451
Publisher
Nature Publishing Group
Year
2007
Tongue
English
Weight
211 KB
Volume
2
Category
Article
ISSN
1750-2799

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The mtDNA (mitochondrial DNA) extracted from etiolated seedlings of Cucumis sativus L. has been purified by a three-step procedure: RNase and pronase treatment, bio-gel filtration, analytical CsCl gradient centrifugation. This procedure appeared rapid, suitable for small quantities of DNA and gave h