To purify the tetrachlorobenzo-p-dioxins synthesized for chemical and biological reference standards, reversed-phase liquid chromatography on pyrenylethyl-and octadecylsilylated stationary phase (PYE and Crs) columns was employed with 100% methanol. The pyrenylethyl phase satisfactorily separated is
Purification of PCDD synthesis products by reversed-phase liquid chromatography with pyrenylethylsilylated and octadecylsilylated silica gel
โ Scribed by Elizabeth R. Barnhart; Donald G. Patterson Jr.
- Book ID
- 119022746
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 196 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0045-6535
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๐ SIMILAR VOLUMES
Recovery of hydrophobic proteins from an RP-HPLC column was improved using a fast-separation RP-HPLC system operated at room temperature. Hydrophobic proteins such as ovalbumin could be adequately eluted from a nonporous octadecylsilyl (C18) spherical silica gel with a particle diameter of 20 micron
The enzymatic syntheses of ATP analogs, such as tubercidin 5 '-triphosphate, formycin A 5 '-triphosphate, and etheno-ATP, from their respective mono- and diphosphate are described. The reaction products were purified by reverse-phase HPLC using a C-18 matrix and a volatile mobile phase at \(\mathrm{