Fast protein separation by reversed-phas
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Hiroko Itoh; Noriyuki Nimura; Toshio Kinoshita; Norikazu Nagae; Mitsugu Nomura
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Article
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1991
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Elsevier Science
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English
โ 388 KB
Recovery of hydrophobic proteins from an RP-HPLC column was improved using a fast-separation RP-HPLC system operated at room temperature. Hydrophobic proteins such as ovalbumin could be adequately eluted from a nonporous octadecylsilyl (C18) spherical silica gel with a particle diameter of 20 micron