Reversed-phase liquid chromatography of the twenty-two tetrachlorodibenzo-p-dioxin isomers on pyrenylethyl- and octadecylsilylated silica gel columns
✍ Scribed by Elizabeth R. Barnhart; Donald G. Patterson Jr.; Nobuo Tanaka; Mikio Araki
- Book ID
- 104145725
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 698 KB
- Volume
- 445
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
To purify the tetrachlorobenzo-p-dioxins synthesized for chemical and biological reference standards, reversed-phase liquid chromatography on pyrenylethyl-and octadecylsilylated stationary phase (PYE and Crs) columns was employed with 100% methanol. The pyrenylethyl phase satisfactorily separated isomers resulting from mixtures of reaction products which had not been adequately separated with conventional Cis or silica gel. The use of a single chromatographic mode, liquid chromatography, and a single mobile phase with columns of distinctly different properties, Cle and PYE, separates 20 of the 22 isomers from each other and from the 1246/1249 pair. (The observed properties of the two columns afford insight into steric and polarizability differences among isomers.) l Use of trade names is for identification only and does not constitute endorsement by the Public Health Service or the U.S. Deuartment of Health and Human Services.