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Purification of N-acetyl β-D-hexosaminidase from bull epididymis by affinity chromatography

✍ Scribed by Miroslav Pokorny; Cornelis P.J. Glaudemans


Book ID
115905984
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
321 KB
Volume
50
Category
Article
ISSN
0014-5793

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Endo-N-acetyl-P-D-glucosaminidase H (Endo H) was purified to homogeneity (3000-fold) from a culture filtrate of Streptomyces plicatus. The key step was substrate-affinity chromatography, which afforded a lOOO-fold purification and yielded a protease-and exoglycosidase-free preparation of Endo H. Pro