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Purification of N-acetyl D-glucosamine-binding proteins by affinity chromatography

✍ Scribed by M.E. Rafestin; A. Obrenovitch; A. Oblin; M. Monsigny

Book ID
115904822
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
355 KB
Volume
40
Category
Article
ISSN
0014-5793

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Purification of endo-N-acetyl-Ξ²-d-glucos
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✍ Urs F. Greber; Branko KozuliΔ‡; Klaus Mosbach πŸ“‚ Article πŸ“… 1989 πŸ› Elsevier Science 🌐 English βš– 815 KB

Endo-N-acetyl-P-D-glucosaminidase H (Endo H) was purified to homogeneity (3000-fold) from a culture filtrate of Streptomyces plicatus. The key step was substrate-affinity chromatography, which afforded a lOOO-fold purification and yielded a protease-and exoglycosidase-free preparation of Endo H. Pro