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Purification of N-acetyl-hexosaminidase from boar epididymis by affinity chromatography

✍ Scribed by E. Bamberg; F. Dorner; W. Stöckl


Book ID
112655109
Publisher
Springer
Year
1975
Tongue
English
Weight
405 KB
Volume
31
Category
Article
ISSN
1420-682X

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Endo-N-acetyl-P-D-glucosaminidase H (Endo H) was purified to homogeneity (3000-fold) from a culture filtrate of Streptomyces plicatus. The key step was substrate-affinity chromatography, which afforded a lOOO-fold purification and yielded a protease-and exoglycosidase-free preparation of Endo H. Pro